Residual Bovine Serum Albumin (BSA)
Determination by ELISA - Non-GLP:
Protocol #: CB127
Sample requirements: 1 ml or one cell pellet shipped overnight on ice packs
Turnaround time: One week, Price: $400 per sample for 1-2 samples per shipment, $350 per sample if 2-4 samples per shipment and $275 per sample if 5 or more samples per shipment.
We offer Residual BSA determination
by ELISA. This assay is performed
using the Bovine Albumin ELISA
kit manufactured by Bethyl Laboratories.
Maxisorp Nunc 96 well plates
are coated with a sheep anti-BSA
antibody. Samples and standards
(400 ng/ml down to 0 ng/ml)
are added to the appropriate
wells and following incubations
and several washes, the conjugate
HRP-tagged antibody is added
to all wells followed by a substrate. Optical
density readings are reduced
using a four parameter logistic
in order to generate a standard
curve and calculate sample residual
BSA concentrations.
This
is an example of a standard
curve obtained in the Residual
Bovine Serum Albumin ELISA Assay. The
sample concentration is deduced
from the four parameter logistic
formula generated in the assay. Standard
range is 0-400 ng/ml
The sponsor will NOT be required to review and approve a protocol prior to study initiation. Please fill out the sample submission form below in either Word or PDF format and send along with the samples.
or
Please contact Dr.
Kilani if you have any
questions.
Doing business with Clongen is
easier than ever!
just download and complete the
sample submission form,
complete the necessary information
and we'll do the rest.
